Journal: International Journal of Oncology

Article Title: Cigarette smoke induces nuclear translocation of heme oxygenase 1 (HO-1) in prostate cancer cells: Nuclear HO-1 promotes vascular endothelial growth factor secretion

doi: 10.3892/ijo.2013.1910

Figure Lengend Snippet: Cigarette smoke induced expression of HO-1 in prostate cancer cells. (A and B) SM induced mRNA levels of HO-1 in prostate cancer cells. DU145 and PC3 were treated with SM for 0, 3 and 6 h. Total RNA was isolated and analyzed by semi-quantitative RT-PCR using primers for HO-1 and GAPDH (internal control). Relative HO-1 mRNA levels derived from three individual experiments were expressed in arbitrary units. Columns, mean; bars, SD; * p<0.05; ** p<0.01. (C and D) SM increased HO-1 protein levels in prostate cancer cells. (C) DU145 and (D) PC3 cells were grown on 6-well plates and treated with SM for 0 and 24 h. Cell extracts were subjected to western blot analysis using anti-HO-1 and anti-GAPDH antibodies. GAPDH served as an internal control. Relative HO-1 protein levels derived from three individual experiments were expressed in arbitrary units. Columns, mean; bars, SD; * p<0.05; ** p<0.01.

Article Snippet: RNA transcript levels were semi-quantified using the Titanium One-Step RT-PCR kit (Clontech) according to the manufacturer’s instructions using the forward 5′-GAGACGGCTTCAAGCTGGTGATG-3′ and reverse 5′-GTTGAGCAGGAACGCAGTCTTGG-3′ primers for HO-1, and the forward 5′-GAAGGTGAAGGTCGGAGTC-3′ and reverse 5′-GAAGATGGTGATGGGATTTC-3′ primers for GAPDH.

Techniques: Expressing, Isolation, Quantitative RT-PCR, Derivative Assay, Western Blot